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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mech Ageing Dev. 2009 Mar 24;130(6):384–392. doi: 10.1016/j.mad.2009.03.002

Figure 6. TNFα is required for Fr. A/NK inhibition of E47 protein levels in B cell precursors.

Figure 6

CD19+ cells were sorted from young BALB/c bone marrow and plated with CD19- B220+ (Fr. A) bone marrow cells (1:5 effector:target ratio) or cell-sorted CD3-CD19- B220+ DX5+ bone marrow cells (1:10 effector:target ratio) in transwells. Control antibody (goat IgG), anti-IFNγ goat polyclonal antibody, or anti-TNFα goat polyclonal antibody were added at the inception of culture at 5μg/ml. Post 7 days of culture with IL-7, the CD19+ cells were harvested and E47 and Ubc9 and/or actin (loading control) proteins detected by Western blot. E47 values were normalized to both loading controls and to values for CD19+ cells cultured alone. Cumulative results are shown for 4 experiments. E47 values were normalized to their respective loading controls and then to young CD19+ values in each experiment with CD19+ E47 values each set to 1. (*p≤0.05 by Student's t-test for the anti-TNFα group vs. control Ab group; NS, not significant for experimental anti-IFNγ group vs. control Ab group).