Table 1.
Primer sequences and PCR strategy used in this study
| Gene | Primer sequencesa | Annealing Temp (°C) |
|---|---|---|
| Human ER α | F, 5'-AGGCTGCGGCGTTCGGC-3' | 53 |
| R, 5'-AGCCATACTTCCCTTGTCAT-3' | ||
| Murine ER α | F, 5'-CGTGTGCAATGACTATGCCTC-3' | 65 |
| R, 5'-TTTCATCATGCCCACTTCGTAA-3' | ||
| Human ER β | F, 5-TTCCCAGCAATGTCACTAACT-3' | 54 |
| R, 5-CTCTTTGAACCTGGACCAGTA-3' | ||
| Murine ER β | F, 5-CTGTGATGAACTACAGTGTTCCC-3' | 62 |
| R, 5-GCAGTGGGTGGCTAAAGGA-3' | ||
| Human PR | F, 5-GATTCAGAAGCCAGCCAGAG-3' | 55 |
| R, 5-AGTTGCCTCTCGCCTAGTTG-3' | ||
| Murine PR | F, 5-GAGCTGCATTCTACTCGCTG-3' | 62 |
| R, 5-CGCGGATATAGCTTGATCTCATC-3' | ||
a
F, Forward primer, R, reverse primer