(A) Results of fluorescence-activated cell sorter analysis using premalignant (1198) and malignant (1170-1) HBE cell lines, and the H1299, H460, and A549 NSCLC cell lines. Each cell line was treated with 100 nM liposomal deguelin or deguelin for 36 hours. Left lower quadrant, unstained cells; left upper quadrant, PI-stained cells (necrotic); right upper quadrant, annexin V and PI-stained cells (late apoptotic cells and necrotic cells); right lower quadrant, annexin V stained cells (early apoptotic cells). (B) Caspase-3 activity was measured at the time points indicated in control and treated cells.