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. Author manuscript; available in PMC: 2009 Sep 14.
Published in final edited form as: J Cell Biochem. 2009 May 15;107(2):364–375. doi: 10.1002/jcb.22136

Fig. 6. Downmodulation of endogenous p130Cas induces apoptosis and sensitizes cells to tamoxifen.

Fig. 6

A: MCF-7 and TAM-R cells were transiently transfected with 80 pmol of p130Cas siRNA as well as with control siRNA. Representative immunoblots (of three independent experiments) demonstrating the downmodulation of Cas expression at 24 h post transfection and phase contrast images of siRNA transfected cells are shown (×10 magnification). B: TAM-R cells were transiently transfected with 80 pmol of p130Cas siRNA as well as with control siRNA for 24 h and then treated with 1 μM Tamoxifen (Tam) and vehicle (ethanol) for an additional 24 h. Cells were then harvested and fixed with 70% ethanol and stained with PI. Cell populations were subjected to flow cytometry as described above. Histograms and percentage of cells in sub-G1 of one experiment are presented (upper panel). The fold changes in sub-G1 were determined by comparing the Cas siRNA treated sets to siRNA treated controls within each group (set to 1) (lower panel). Data represent three independent experiments performed in triplicate (± standard deviation). P values were calculated using Student’s t-test.