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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Nat Med. 2009 Aug 23;15(9):1077–1081. doi: 10.1038/nm.2005

Figure 4. RNAi-mediated knockdown of NOX4 attenuates fibrosis in mice subjected to fluorescein isothiocyanate-induced lung injury.

Figure 4

C57BL/6 mice were administered intra-tracheal fluorescein isothiocyanate (FITC) or saline/control on day 0 with nontargeting control siRNA or NOX4 siRNA and lung tissues were analyzed on day 14 or 21. (a) NOX4 expression on day 21 was determined by Western immunoblotting of whole lung homogenates. (b) Fibrosis was assessed by H & E staining and Masson’s trichrome blue staining for collagen; length bar = 100 μm. (c,d) Whole lung homogenates were analyzed on day 14 for acid-soluble collagen using the Sircol assay (c), and on day 21 for hydroxyproline content (d). Values represent mean± S.E.M.; n = 4–6; *P < 0.01 compared to saline, nontargeting (control) siRNA.