Fig. 3.

Transcription activity of 15-LOX-1 promoter deletion constructs. (A) SW480 cells and (B) Caco-2 cells were transiently transfected with PGL4.16 luciferase reporter vectors containing the −120 to +18 bp (−120), −391 to +18 bp (−391), or −3500 to +18 bp (−3500) regions of the 15-LOX-1 promoter and treated with either depsipeptide or the control vehicle. Transcription activity was measured 24 h after treatment. (C and D) PGL4.16 vectors containing −120, −391, or −729 to +18 bp (−729) regions of the 15-LOX-1 promoter were stably transfected into SW480 cells. (C) Basal transcription levels (measured as luciferase reporter activity) for representative stably transfected clones with −120, −391, and −729 region vectors are shown. Values are the means ± SDs of triplicate experiments. (D) Depsipeptide effects on transcription activation for the −120 and −391 regions of the 15-LOX-1 promoter. SW480 stably transfected clones with −120 or −391 region vectors (as described in panel C) were treated with either depsipeptide or DMSO only (control). Luciferase activity was measured 24 h later. Values are the ratios of depsipeptide- to control-treated cells (means ± SDs of triplicate experiments). * P < 0.0001.