Table 2. Comparison of FCM-DNA and ICM-DNA for different DNA ploidy category definitions: (A) traditional DNA diploid vs DNA non-diploid category; (B) DNA diploid vs DNA tetraploid vs DNA aneuploid; (C) DNA non-aneuploid (DNA diploid or DNA tetraploid) vs DNA aneuploid; (D) ICM-DNA category i: (DNA diploid or DNA tetraploid) and 9c exceeding rate of 0 vs category ii: DNA aneuploid or ((DNA diploid or DNA tetraploid) and 9c exceeding rate >0) vs FCM-DNA DNA non-aneuploid (DNA diploid or DNA tetraploid) vs DNA aneuploid; (E) Percentage of concordant results, P-value obtained using either the χ2 or Fisher's exact test and the κ-values for Tables 2A–D.
| FCM-DNA | ||||
|---|---|---|---|---|
| (A) | Diploid | Non-diploid | Total | |
| ICM-DNA | ||||
| Diploid | 27 (12.2%) | 22 (10.0%) | 49 | |
| Non-diploid | 16 (7.2%) | 156 (70.6%) | 172 | |
| Total | 43 | 178 | 221 | |
|
FCM-DNA
|
||||
| (B) | Diploid | Tetraploid | Aneuploid | Total |
| ICM-DNA | ||||
| Diploid | 27 (12.2%) | 1 (0.5%) | 21 (9.5%) | 49 |
| Tetraploid | 5 (2.3%) | 3 (1.4%) | 9 (4.1%) | 17 |
| Aneuploid | 11 (5.0%) | 6 (2.7%) | 138 (62.4%) | 155 |
| Total | 43 | 10 | 168 | 221 |
|
FCM-DNA
|
||||
| (C) | Diploid+tetraploid | Aneuploid | Total | |
| ICM-DNA | ||||
| Diploid+tetraploid | 36 (16.3%) | 30 (13.6%) | 66 | |
| Aneuploid | 17 (7.7%) | 138 (62.4%) | 155 | |
| Total | 53 | 168 | 221 | |
|
FCM-DNA
|
||||
| (D) | Diploid+tetraploid | Aneuploid | Total | |
| ICM-DNA | ||||
| Category 1 | 35 (15.8%) | 26 (11.8%) | 61 | |
| Category 2 | 18 (8.1%) | 142 (64.3%) | 160 | |
| Total | 53 | 168 | 221 | |
| (E) | Concordance (% cases) | Pearson's χ2 P-value | κ-value | |
| Category definition | ||||
| A | 82.8 | P<0.001 | 0.48 | |
| B | 76 | P=0.03* | 0.43 | |
| C | 78.7 | P<0.001 | 0.46 | |
| D | 80.1 | P<0.001 | 0.48 | |
Abbreviations: FCM-DNA, DNA ploidy status measured using flow cytometry; ICM-DNA, DNA ploidy status measured using image cytometry.
*P-value obtained using Fisher's exact test.