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. 2009 Aug 4;101(6):983–991. doi: 10.1038/sj.bjc.6605236

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Copyright © 2009 Cancer Research UK

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SDF-1-α-mediated invasion is higher in HSB-2 cells than in CD26-depleted clones. (A) Cell-surface expression of CD26 on HSB-2 cell lines. (a) HSB-2 parental (b) H1-2 (c) 2E5 (d) 2F8 (e) 2G9. Left: cells incubated with PE-labeled isotypic control IgG. Right: cells incubated with PE-labeled anti-CD26 antibody. (B) Expression of CD26 in whole-cell extracts. Equal amounts of protein were run in each lane of a 7.5% gel, transferred to nitrocellulose, and probed with anti-CD26 antibody. Lanes contained HSB-2 parental cells, H1-2, and four clones depleted of CD26: 2E5, 2F8, 2F10, and 2G9. Note that CD26 runs as a dimer (see Materials and Methods). The multiple bands are due to differences in glycosylation. (C) Invasion assay (details in Materials and Methods). Assays were carried out for 24 h, at which time the cells were counted in all wells. Percentage invasion is the number of cells which passed through the transwell divided by the total cell number. Error bars are shown for the standard error of the mean.