Figure 4.

SDF-1-α induces phosphorylation of signalling proteins in T cell lines. (A) Activation of signalling kinases in HSB-2 cells following incubation with SDF-1-α. Cells were incubated with 10 nM SDF-1-α for the indicated times, run on gels, transferred to nitrocellulose, then probed with site-specific antibodies. Top left, phosphorylated MAPK p44/42 (thr202/tyr204); bottom left, phosphorylated Akt (Ser473). Data depicted are representative of two separate experiments. (B) Activation of signalling kinases in Jurkat wt1. Top right, phosphorylated MAPK p44/42 (thr202/tyr204); bottom right, phosphorylated Akt (Ser473). Data depicted are representative of three separate experiments. Total p44/42 MAPK and Akt are shown below their respective phosphorylated kinases.