Table 1. Invasion is inhibited in the presence of kinase inhibitors.
Inhibitor | Inhibition for wt1 (%) | Inhibition for H1-2 (%) |
---|---|---|
LY294002 | 70.4 | 48.5 |
PD98059 | 50.0 | 35.4 |
SB203580 | 12.8 | 23.8 |
Cells were incubated with inhibitors for 1 h before the start of invasion assays. The phosphatidylinositol 3-kinase (PI-3K) inhibitor, LY294002, and the MEK1 inhibitor, PD98059, were used at a concentration of 50 μM, whereas the p38 MAP kinase inhibitor, SB203580, was used at a concentration of 10 μM. Invasion level was determined following 24 h of incubation in the presence of SDF-1-α. Per cent inhibition=per cent invasion in the absence of inhibitor−per cent invasion in the presence of inhibitor/per cent invasion in the absence of inhibitor. Data depicted are representative of two separate experiments.