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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Struct Biol. 2009 Mar 6;166(3):316–328. doi: 10.1016/j.jsb.2009.02.014

Figure 2.

Figure 2

Comparison of conventional SEM and ion-abrasion SEM imaging of T. pseudonana. (a and b) Surface imaging of T. pseudonana emphasizing (a) the entire cell structure, and (b) a close-up of the valve surface. (c and d) Cross-sectional images of T. pseudonana generated by ion-abrasion SEM. In both images silica surrounding the cell is highly electron bright and intracellular membranes are less bright. Numerous vacuoles are visible, and in (d), thylakoid membranes of the plastid are predominant. (e) Surface image of the proximal valve surface of T. pseudonana. Note the smooth but ribbed internal structure and inner projections of the rimoportulae. (f) 3D reconstruction of a series of images of the proximal valve surface of T. pseudonana generated by ion-abrasion SEM. Similar features are visible as in the surface image in (e).