Table 1.
Primer sequences used for real-time RT-PCR determinations. Accession: GeneBank accession number for sequences publicly available at http://www.ncbi.nlm.nih.gov/sites/entrez. IL-4: interleukin-4; IL-5: interleukin-5; IL-13; interleukin-13; CRF: corticotropin releasing factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; ACTB: actin, beta isoform; 18S: ribosomal RNA subunit 18S; mus: mus musculus; rattus: rattus norvegicus
| Gene (specie) | Accession | Primer sequence | Region | Product length | |
|---|---|---|---|---|---|
| IL-4 (mus) | NM_021283 | Fwd | 5’AGAACACCACAGAGAGTGAG3’ | 266-417 | 152 |
| Rev | 5’TGAATCCAGGCATCGAAAAG3’ | ||||
| IL-13 (mus) | NM_008355 | Fwd | 5’TCTTGCTTGCCTTGGTGGTC3’ | 98-237 | 140 |
| Rev | 5’ATACCATGCTGCCGTTGCAC3’ | ||||
| IL-5 (mus/rattus) | NM_010558 (mus) | Fwd | 5’CATGAGCACAGTGGTGAAAG3’ | 115-252 (mus) | 138 |
| NM_021834 (rattus) | Rev | 5’AGCCCCTGAAAGATTTCTCC3’ | 69-206 (rattus) | ||
| CRF (mus/rattus) | NM_205769 (mus) | Fwd | 5’GGATCTCACCTTCCACCTTC3’ | 633-734 (mus) | 102 |
| NM_031019 (rattus) | Rev | 5’CCGATAATCTCCATCAGTTTCC3’ | 631-732 (rattus) | ||
| GAPDH (mus) | NM_008084 | Fwd | 5’CCACTCACGGCAAATTCAAC3’ | 196-338 | 142 |
| Rev | 5’AGACTCCACGACATACTCAG3’ | ||||
| ACTB (mus) | NM_007393 | Fwd | 5’TGAGACCTTCAACACCCCAG3’ | 452-591 | 140 |
| Rev | 5’GAGCATAGCCCTCGTAGATG3’ | ||||
| 18S (mus/rattus) | NR_003278 (mus) | Fwd | 5’CCAGTAAGTGCGGGTCATAAGC3’ | 1645-1727 (mus) | 83 |
| X01117 (rattus) | Rev | 5’CCATCCAATCGGTAGTAGCGAC3’ | 1650-1732 (rattus) | ||
| IL-4 (rattus) | NM_201270 | Fwd | 5’TTACGGCAACAAGGAACAC3’ | 197-336 | 140 |
| Rev | 5’ACAGAGTTTCCTCAGTTCACC3’ | ||||
| IL-13 (rattus) | NM_053828 | Fwd | 5’GCTGAGCAACATCACACAAG3’ | 117-279 | 162 |
| Rev | 5’GAGGCCATTCAATATCCTCTG3’ | ||||
| GAPDH (rattus) | NM_017008 | Fwd | 5’TACCCACGGCAAGTTCAACG3’ | 223-362 | 140 |
| Rev | 5’ACTCCACGACATACTCAGCAC3’ | ||||
| ACTB (rattus) | NM_031144 | Fwd | 5’TGAGACCTTCAACACCCCAG3’ | 453-592 | 140 |
| Rev | 5’GCGCGTAACCCTCATAGATG3’ | ||||