Skip to main content
. Author manuscript; available in PMC: 2009 Sep 14.
Published in final edited form as: Glycoconj J. 2008 Dec 17;26(5):511–523. doi: 10.1007/s10719-008-9207-8

Fig 5.

Fig 5

Binding of HECA-452 antibody to CHO/PSGL/C2/F7 cells before and after stable expression of CMP-NeuAc hydroxylase. CHO cells expressing PSGL-1, Core2GlcNAcT, FucT-VII were stably transfected with CMP-NeuAc hydroxylase. Resultant cell lines (clones 5 and 11) and the parental line were subjected to flow cytometry analysis using HECA-452 antibody. Binding of HECA-452 antibody was detected by FITC-conjugated secondary antibody (solid lines). Mouse IgM served as a control (dotted lines). Cell lines expressing N-glycolylneuraminic acid (clones 5 and 11) were negative for HECA-452 staining, while the parental cell line was positive