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Expression of SOX9 in wild-type livers and in livers with conditional inactivation of Sox9 alleles. (A) Expression profiling by immunostaining showed that SOX9 is an early and specific marker of the developing bile ducts. (B) In Alfp-Cre-Sox9loxP/loxP (Sox9 knockout) embryos, the livers were depleted of SOX9 starting at e11.5, except for one individual showing residual SOX9+ cells at e13.5 (arrow). hb, hepatoblasts; ld, liver diverticulum; pv, portal vein.
