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. Author manuscript; available in PMC: 2010 Aug 12.
Published in final edited form as: Structure. 2009 Jul 2;17(8):1082–1091. doi: 10.1016/j.str.2009.06.003

Figure 5. Nup1201-757 Does Not Localize to the Nuclear Envelope.

Figure 5

(Aa-Ad) Nup120-GFP is targeted to the nuclear envelope, as confirmed by co-localization with mAb414 (staining FG-Nups), while Nup1201-757-GFP (Ba-Bd) is distributed throughout the cell. Mislocalization indicates that the C-terminus of Nup120 is necessary for proper recruitment to the NPC. Nup120Δ, nup133Δ, and nup84Δ cells (in the same BY4741 strain background) are shown for comparison (Cb-Cd, Db-Dd, Eb-Ed). Nuclear rim was visualized using mAb414, GFP-tagged Nup120 using goat α-GFP, and DNA using DAPI. Merged images are shown on the right.