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FACT localizes to centromeres. Localization of endogenous SSRP1 and SPT16 in CENP-H-RFP–expressing cells was visualized using anti-SSRP1 and anti-SPT16 antibodies. (A) Cells fixed with paraformaldehyde. (B) Cells treated with 0.1% Triton X-100 in PBS for 2 min before paraformaldehyde fixation. SSRP1 and SPT16 (green), CENP-H-RFP (red), and DAPI (blue) staining are shown.
