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Depletion of FACT does not affect localization of centromere proteins. Immunostaining of SSRP1 conditional mutant cells with anti-CENP-H, anti-CENP-K and anti-CENP-C antibodies. Cells were cultured for 45 h in the presence of tet (+Tet). Immunostaining signals were detected with fluorescein isothiocyanate (FITC)-conjugated secondary antibodies (green). DNA was counterstained with DAPI (blue).
