Figure 2.

(A) Schematic representation of potential Dlk phosphorylation sites in the Par-4 protein. Full-length rat Par-4 (Par-4 wt, 332 amino acids) contains two nuclear localization signals (NLS1 and NLS2), and a leucine zipper motive (LZ) located within the death domain (DD) at the carboxy-terminus (shaded box). The putative Dlk phosphorylation sites in the rat Par-4 protein are indicated. (B) Sequence comparison of known Dlk substrates. The positions of the required arginine residue at the −3 position preceding the phospho-acceptor site are highlighted in all substrates, suggesting a minimal Dlk consensus motif of R-X-X-(S/T).