Figure 5.

Two distinct subpopulations of the Nup107-160 complex in egg cytosol differ in ELYS content. Xenopus egg cytosol was subfractionated on a DEAE Affi-Gel Blue column followed by a Q-Sepharose column. The second column was eluted with a linear 0.1–1 M NaCl gradient. The column elution profile is shown by OD₂₈₀ absorbance (in blue). Fractions 26–34, from the region containing ELYS and Nup107-160 complex members, were analyzed by immunoblotting as shown in the bottom panel. The blot was probed with antibodies to ELYS and Nup107-160 complex members. Only fractions 29–31, eluted at 200–230 mM NaCl, contained significant amounts of ELYS, whereas Nup107-160 complex members peaked over the whole 200–260 mM NaCl range (fractions 29–34).