Figure 1.

NO is involved in transducing responses to hypoxia. (A) S2 cells expressing GFP-Rel were either left untreated (normoxia, N) or cultured in 1% O₂ for 8 h (hypoxia, H) or in the presence of SNAP (2 mM, S) for 3 h before fixation and analysis for GFP localization. For illustration, insets represent a higher magnification of a cell in the image. (B) Left, GFP-Rel cells were either left untreated (con), treated with PTIO (0.2 mM, 30 min), L-NAME (1 mM, 30 min), L-NAME (1 mM, 48 h), D-NAME (1 mM, 48 h), or with dsRNAi against NOS (5 d) before and during o/n incubation under normoxic circumstances (normoxia), or hypoxia (1% O₂). In this and subsequent figures, the bars are averages of at least three independent experiments; error bars, ±SEM. Right, RNAi against NOS knocks down NOS mRNA. Cells were treated without or with NOS dsRNA, and expression of NOS was analyzed by RT-PCR. RP49 was used as a control for equal input.