Figure 2.

(A) Scavenging of nitric oxide in the mitochondria attenuates responses to hypoxia. Localization of NOR constructs. S2 cells were transfected with NOR-HA or mtNOR-HA construct and stained with mitotracker-Red (50 nM) for 30 min before fixation and staining with anti-HA (green) and DNA staining (Hoechst, blue). (B) S2 cells were cotransfected with GFP-Rel and either empty vector (con), NOR-HA, mtNOR-HA, inactive NOR-HA or inactive mtNOR-HA and analyzed for HA expression by Western blot. (C) Analysis of samples in B for GFP-Rel localization in normoxia or after NO treatment (SNAP, 2 mM, 3 h) or hypoxia (8 h, 1% O₂).