Table 4.
Virus infection dose (TCID50) | No. with positive titer in lung/total no. (Log10TCID50/lung ± SD) |
5 × 105 | 3/3 (5.6 ± 0.4) |
5 × 104 | 3/3 (5.2 ± 0.3) |
5 × 103 | 3/3 (4.5 ± 0.4) |
500 | 3/3 (3.7 ± 0.6) |
50 | 0/3 |
a 4–5 week old female BALB/c mice were anesthetized by isoflurane and infected i.n. with the RGMal/01 virus at a dose 50, 500, 5.0 × 103, 5.0 × 104, 5 × 105 TCID50/50 μl. At day 3 post-inoculation, lungs from three infected mice were collected and homogenized to titrate the virus by TCID50 assay in MDCK cells.