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. 2009 Aug 12;9:281. doi: 10.1186/1471-2407-9-281

Figure 3.

Figure 3

DRB-induced apoptosis is mediated by cytosolic p53 accumulation and Bax activation. (A) Confocal analysis of p53 localization. Control T cells were harvested before or 6 hr after 100 μM DRB treatment and stained for p53 and with green MitoTraker as cytosolic marker. One representative experiment out of three is shown. (B) Immunoblot analysis of p53, p53pSer15 and Bax localization. Time course analysis of cytosolic and mitochondrial fractions on control T cells after 100 μM DRB treatment. One representative experiment out of two is shown. Positive control (+ lane): whole cell lysate from a control T cell line treated for 6 hr with 0,05 μg/ml ActD. (C) Confocal analysis of Bax distribution. Control T cells were harvested before or 6 hr after 100 μM DRB and stained for Bax and with green MitoTraker as cytosolic marker. One representative experiment out of three is shown. (D) Survival analysis of DRB-treated T cells after p53 inhibition. Control T cells were treated with either α-PFT or μ-PFT alone or with 100 μM DRB and analyzed at 24 hr by flow cytometry after stainig with PI and (E) with anti-active caspase-3 antibody. Data are normalized to caspase activation with DRB alone (mean ± S.D.).