Table 1.
List of transcripts modulated by DIMEB and reported in the Discussion
IDa | Description | Uniprot IDb | TC-IDc | 2 h | 6 h | |
+ | + | - | ||||
Signal trasduction | ||||||
CLU090 | Kinase associated protein phosphatase | P46014 | EC987592 | x | ||
1608981_at | Putative phospholipase | Q8RXN7 | TC69626 | x | ||
1620080_at | Putative receptor-like protein kinase ARK1 | Q5ZAK8 | CB922377 | x | ||
1611172_at | SOS2-like protein kinase | Q8LK24 | TC52484 | x | ||
Transcription factors | ||||||
1619311_at | Pathogenesis-related genes transcriptional activator PTI5 | O04681 | TC55556 | x | ||
1611285_s_at | Probable WRKY transcription factor 11 | Q9SV15 | TC65678 | x | ||
CLU059 | TGA10 transcription factor | Q52MZ2 | TC99087 | x | ||
1610775_s_at | WRKY transcription factor-b | Q5DJU0 | TC55553 | x | ||
Effector genes | ||||||
Phe biosynthesis | ||||||
CLU083 | 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase precursor | O24051 | TC74975 | x | ||
1611211_at | 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase precursor | O24046 | TC57386 | x | ||
1614440_at | 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase | Q6YH16 | TC54321 | x | ||
1619357_at | 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase | O24046 | TC57642 | x | ||
1621405_at | Plastidic 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 2 | O22407 | TC51974 | x | ||
1609646_at | 3-Dehydroquinate synthase-like protein | Q9FKX0 | TC56854 | x | ||
1609932_at | Prephenate dehydratase | Q6JJ29 | TC53641 | x | ||
1621307_at | Prephenate dehydratase | Q6JJ29 | TC53641 | x | ||
1611895_at | Putative chorismate mutase | Q5JN19 | TC62307 | x | ||
General phenylpropanoid metabolism | ||||||
1613113_at | Phenylalanine ammonia lyase | Q6UD65 | TC60180 | |||
CLU024 | Trans-cinnamate 4-monooxygenase | Q43240 | TC71512 | x | ||
1610821_at | Cinnamic acid 4-hydroxylase | Q948S8 | TC70715 | x | ||
1616191_s_at | Cinnamic acid 4-hydroxylase | Q948S8 | TC70715 | x | ||
1615801_at | 4-Coumarate:CoA ligase | Q5S017 | TC60943 | x | ||
1619320_at | 4-Coumarate--CoA ligase 2 | P31687 | TC66743 | x | ||
Stilbene biosynthesis | ||||||
CLU009 | Stilbene synthase | Q9SPW2 | TC89701 | x | ||
CLU022 | Stilbene synthase | Q6BAU9 | TC89632 | x | ||
CLU023 | Stilbene synthase | P28343 | TC84974 | x | ||
CLU049 | Stilbene synthase | Q8LPP4 | TC78210 | x | ||
CLU097 | Stilbene synthase | Q9S982 | TC84974 | x | ||
CLU103 | Stilbene synthase | P28343 | TC88894 | x | ||
1606750_at | Stilbene synthase | Q6BAL2 | TC67020 | x | ||
1608009_s_at | Stilbene synthase | P51070 | x | |||
1609696_x_at | Stilbene synthase | P28343 | TC67020 | x | ||
1609697_at | Stilbene synthase | Q944W7 | TC60946 | x | ||
1610824_s_at | Stilbene synthase | Q93YX5 | TC52746 | x | ||
1610850_at | Stilbene synthase | P28343 | x | |||
1611190_s_at | Resveratrol synthase | Q94G58 | TC67020 | x | ||
1612804_at | Stilbene synthase | Q9SPW2 | TC52746 | x | ||
1614621_at | Stilbene synthase | P28343 | TC67020 | x | ||
1616575_at | Stilbene synthase | Q944W7 | TC52746 | x | ||
1620964_s_at | Stilbene synthase | P28343 | x | |||
1622638_x_at | Stilbene synthase | Q9SPW2 | TC52746 | x | ||
Secondary metabolite transport | ||||||
CLU106 | PDR-like ABC transporter | Q8GU88 | TC76318 | x | ||
CLU119 | Pleiotropic drug resistance protein 12 | Q5Z9S8 | TC81892 | x | ||
1613763_at | ABC transporter-like protein | Q9LYS2 | TC60768 | x | ||
1618493_s_at | ABC transporter-like protein | Q9LYS2 | TC64210 | x | ||
1610363_at | CjMDR1 | Q94IH6 | TC69843 | x | ||
1609330_at | Glutathione S-transferase | Q6YEY5 | NP864091 | x | ||
1611890_at | Glutathione S-transferase GST 14 | Q9FQE4 | TC61062 | x | ||
1619682_x_at | Caffeic acid O-methyltransferase | Q9M560 | TC52364 | x | ||
1620342_at | Caffeic acid 3-O-methyltransferase 1 | Q00763 | TC64352 | x | ||
Lignin biosynthesis | ||||||
1611897_s_at | Caffeoyl-CoA O-methyltransferase | Q8H9B6 | TC63685 | x | ||
1614643_at | Caffeoyl-CoA O-methyltransferase | Q43237 | TC51729 | x | ||
1613900_at | Cinnamyl alcohol dehydrogenase | Q9ATW1 | TC52904 | x | ||
1614045_at | Ferulate 5-hydroxylase | Q6IV45 | TC64493 | x | ||
1614502_at | Ferulate 5-hydroxylase | Q6IV45 | TC63764 | x | ||
1619065_at | Putative cinnamoyl-CoA reductase | Q8W3H0 | TC53437 | x | ||
1622651_at | Polyphenol oxidase | Q68NI4 | TC58764 | x | ||
1610806_at | Putative diphenol oxidase | Q6Z8L2 | CD007812 | x | ||
CLU122 | Chalcone-flavonone isomerase | P51117 | TC78712 | x | ||
CLU048 | Flavonol 3-O-glucosyltransferase 6 | Q40288 | TC85607 | x | ||
1621051_at | Flavonol 3-O-glucosyltransferase 2 | Q40285 | CN006197 | x | ||
Defence response | ||||||
CLU088 | Chitinase (Class II) | Q43322 | TC95665 | x | ||
1613871_at | Class IV chitinase | Q9M2U5 | TC57889 | x | ||
1617192_at | Class IV chitinase | Q7XB39 | TC63731 | x | ||
1617430_s_at | Basic endochitinase precursor | P51613 | TC51704 | x | ||
CLU001 | Pathogenesis-related protein10 | Q9FS42 | TC72098 | x | ||
1610011_s_at | Pathogenesis-related protein10 | Q9FS42 | x | |||
1618568_s_at | Pathogenesis-related protein10 | Q9FS42 | x | |||
CLU021 | Pathogenesis-related protein PR-4A precursor | P29062 | TC91296 | x | ||
CLU036 | Merlot proline-rich protein 2 | Q6QGY1 | TC85591 | x | ||
1609875_at | Protease inhibitor | Q6YEY6 | x | |||
1611666_s_at | Protease inhibitor | Q6YEY6 | TC70006 | x | ||
1612552_at | Putative S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase | Q9C9W8 | TC57170 | x | ||
1620309_at | Putative S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase | Q9C9W8 | TC63451 | x | ||
1622147_at | 1-Aminocyclopropane-1-carboxylate oxidase 3 | Q08507 | TC60326 | x | ||
1616358_at | MLO-like protein 11 | Q9FI00 | BQ798612 | x | ||
Cell wall metabolism | ||||||
1608074_s_at | Expansin | Q84UT0 | TC62965 | x | ||
1620840_at | Alpha-expansin | Q8LKJ8 | TC53122 | x | ||
1615995_at | Xyloglucan endotransglycosylase XET2 | Q9LLC2 | CF212592 | x | ||
1620003_at | Xyloglucan endotransglycosylase 1 | Q9ZRV1 | TC63269 | x | ||
1608799_at | Pectin methylesterase | Q96497 | TC58800 | x | ||
1619468_at | Pectin methylesterase PME1 | Q94B16 | TC53043 | x | ||
1619522_at | Putative beta-galactosidase BG1 | Q94B17 | TC56838 | x | ||
1608756_at | Polygalacturonase-like protein | Q84LI7 | TC59719 | x | ||
1606763_at | Putative beta-1,3-glucanase | Q8L868 | TC67051 | x | ||
1609506_at | Putative cellulase CEL2 | Q94B13 | NP596365 | x | ||
1610263_at | Putative beta-1,3-glucanase | Q8L868 | TC67051 | x | ||
Cell duplication | ||||||
1612320_a_at | Tubulin alpha chain | P33629 | TC57547 | x | ||
1616815_at | Tubulin beta-8 chain | Q41785 | TC55048 | x | ||
1618413_at | Tubulin alpha chain | P33629 | TC63601 | x | ||
1619167_at | Tubulin beta-8 chain | Q41785 | TC62643 | x | ||
1621015_at | Alpha-tubulin 1 | Q8H6M1 | TC65238 | x | ||
1622466_at | Tubulin beta-8 chain | Q41785 | TC62809 | x | ||
1608927_at | Putative histone H2A | Q6L500 | TC53574 | x | ||
1612573_at | Histone H3 | A2Y533 | TC56731 | x | ||
1613041_at | Histone H4 | Q76H85 | TC61904 | x | ||
1613076_at | Histone H4 | Q76H85 | TC62637 | x | ||
1620332_at | Histone H3 | A2Y533 | TC59489 | x | ||
1622440_at | Histone H3 | A2Y533 | TC64779 | x | ||
1622737_at | Histone H2B | O22582 | TC64405 | x | ||
1610854_at | Proliferating cell nuclear antigen | P22177 | TC54817 | x | ||
1610422_at | Patellin-6 | Q9SCU1 | TC61622 | x | ||
1610607_at | Gip1-like protein | Q93WR4 | TC66111 | x | ||
1613373_at | Formin-like protein 1 | Q8S0F0 | TC55249 | x | ||
1607792_at | Putative DNA polymerase alpha catalytic subunit | O48653 | TC59012 | x |
aCluster or Affy ID of transcripts modulated at 2 or 6 h. (+) and (-) refer to up- and down-regulation in the treated sample with respect to the control.
bUniprotID [73]of the first hit obtained by “Blast” analysis.
cTC: corresponding grapevine Tentative Consensus sequence obtained by a search (BlastN) against the Grape Gene Index database [75].