Fig. 3.

Dendrite and spine alterations as a function of age in the APP/PS1 and WT mouse hippocampus. (A) APP/PS1 (black bars) and WT (gray bars) mice at 1, 4, and 14 months of age (three per genotype) were shot with DiI-labeled particles and subsequently quantified. An average of 16 dendritic segments were measured for each time point, per genotype, and a total of 2878 spine morphologies were measured (*, P < 0.05, **, P < 0.005, ***, P < 0.0001). (B) Examples of dendrite and spine morphologies of pyramidal hippocampal neurons used for quantification in 14-month-old APP/PS1 and WT mice. WT mice have considerably more spines than APP/PS1 mice, and total dendrite area and dendrite diameter are also increased in the WT animals. Although the transgenic animals have fewer spines, the remaining spines are larger. Scale, 10 μm.