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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Feb 20;1791(10):975–982. doi: 10.1016/j.bbalip.2009.02.002

Figure 8. Inhibition of PKC blocks synergy-enhanced AA release, but does not reduce the synergistic activation value.

Figure 8

A, AA release was measured from RAW 264.7 macrophages preincubated with and without 5 μM Ro 31-8220 prior to stimulation with and without 100 ng/mL Kdo2-Lipid A for 50 minutes and with and without 2 mM ATP for 10 minutes (as indicated in the legend). The media was collected at 1 hour following the addition of the 100 ng/mL Kdo2-Lipid A agonist and analyzed for AA release. B, Synergistic activation values were calculated for uninhibited cells and those preincubated with 5 μM Ro 31-8220. A representative experiment of three individual experiments is shown. Data are expressed as mean values ± S.D. of three individual replicates.