Figure 3.
Quantitative targeted peptidomics of α-MSH and γ-MSH. Peak areas of the MRM transitions are combined and plotted on a log scale for each mouse. Mice are sorted by genotype with the PREPgt/gt mice on the left side and the wild-type PREPWT/WT mice on the right. Linear correlation between the two endogenous peptides underlines that γ-MSH, which is not a putative PREP substrate, can be used as an endogenous control to normalize α-MSH quantities across biological replicates.