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. Author manuscript; available in PMC: 2010 Oct 2.
Published in final edited form as: J Mol Biol. 2009 Jul 23;392(4):885–894. doi: 10.1016/j.jmb.2009.07.052

Figure 2.

Figure 2

Southern blot analysis of DNA extruded from control and formaldehyde-treated C capsids. As described in Materials and Methods, BamHI digests of the extruded DNA were subjected to agarose gel electrophoresis, blotting and probing with the BamHI K fragment (containing both Q and S fragments). The Q and S regions of the gel are shown in (a). Quantitative determination of the Q: S1+S2 ratio is shown below the blot. Note that Q fragments are enriched in the digests of DNA extruded from formaldehyde-treated compared to control capsids. The genomic locations of BamHI Q (3.4 kbp), S (S1: 2.9 kbp; S2 3.3 kbp) and K (6.1 kbp) fragments are shown in (b). S1 and S2 differ in the number of a sequences present; S1 has one and S2 has two.53