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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Neurochem. 2009 Feb 2;109(1):214–224. doi: 10.1111/j.1471-4159.2009.05949.x

Figure 2.

Figure 2

Estradiol promotes nNOS/NR2B complex formation in neurons of the preoptic region in vitro. Treatment of preoptic region neuronal cultures with 17-b-estradiol (E2) results in an increased physical association of nNOS with NR2B. Neurons were cultured in neuronal defined medium and were exposed to E2 1 nM for 30 min or 48 h or to ethanol for control cultures (CTL). Upper panel, 500 μg of proteins per condition were immunoprecipitated (IPP) with a specific nNOS antibody, electrophoresed and immunobloted (IB) for NR2B. Then the immunoblot was stripped and reprobed with antibodies to nNOS to ensure that equal amounts of nNOS protein had been immunoprecipitated and loaded on the gel. Lower panel, bar graph showing the quantitative analysis of the differential NR2B/nNOS association among E2 treatment (n = 4 independent experiments; * p = 0.025 vs CTL). Error bars indicate SEM. Statistical differences were established using a one way ANOVA by the Student-Newman-Keuls multiple comparison test.