Figure 6.

Denbinobin increased the Fas ligand (FasL)-mediated cleavage of caspase-8 and caspase-3 in BxPC-3 cells. Cells were incubated with soluble FasL (sFasL) (50 ng·mL⁻¹), denbinobin (3 µmol·L⁻¹) or both as indicated for 24 h. Cells were harvested and prepared for quantification of the protein levels of caspases-8, -3, -9 and GAPDH (internal control) by Western blot analysis. The numbers below each record are the mean quantitative data obtained by a densitometer. The results shown are representative of three independent experiments.