Skip to main content
. 2009 Jun 25;157(7):1250–1262. doi: 10.1111/j.1476-5381.2009.00297.x

Figure 7.

Figure 7

TAK-242 has no effect on TLR4 homodimerization. (A) Effect of TAK-242 on constitutive TLR4 homodimerization induced by overexpresssion of TLR4-Bla(a) and TLR4-Bla(b). HEK293 cells were transiently transfected with TLR-Bla fusion constructs. On the following day, cells were incubated with test compounds for 3 h. Cells were then loaded with CCF2/AM and analysed by an IN cell analyser 1000. Results are shown as the mean ± SEM (n= 30). (B) Effect of TAK-242 on constitutive NF-kB activation induced by overexpression of TLR4-Bla(a) and TLR4-Bla(b). HEK293 cells were transiently transfected with TLR-Bla fusion constructs. After 6 h of transfection, cells were incubated with various concentrations of test compounds overnight. Luciferase activity was determined using a luciferase assay kit. Data represent the mean ± SEM (n= 6). HEK, human embryonic kidney; NF-κB, nuclear factor-κB; TLR, Toll-like receptor.