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Abnormal development of axon cone development in amacrine neurons growing in insulin-lacking medium. Phase (C) and fluorescence (A, B) photomicrographs showing tubulin (A) and nuclei (B) labeling in day 6 neuronal cultures. In the absence of insulin many amacrine neurons (open arrows) developed wide axon cones (thin white arrows) and tubulin clumps in their neurites, as occurred with photoreceptors.
