Fig. 1.

The 2D ¹³C homonuclear through-bond DQ/SQ spectrum of native (dry) Nephila clavipes spider dragline silk obtained with the refocused INADEQUATE pulse sequence. Data were collected on an 800 MHz spectrometer with a 1 ms CP contact time and a 40 kHz MAS frequency. The spectral width was 62.5 kHz in both dimensions and 256 t₁ increments were collected in the indirect dimension with a 3 s recycle delay. The connectivity and assignment agrees with previous ¹³C correlation spectra collected with through-space dipolar recoupling.⁷