Skip to main content
. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Evol Dev. 2007 Mar–Apr;9(2):131–140. doi: 10.1111/j.1525-142X.2007.00144.x

Fig. 1.

Fig. 1

Artemia franciscana Netrin (afrnet) encodes a netrin orthologue (A). The predicted Artemia franciscana Netrin (afrNet) sequence is aligned to the Drosophila melanogaster NetA (DmNetA) sequence. Regions corresponding to the primers used in this study (FP1, RP6, RP7, and GSP1, see methods for more details) are marked. Conserved amino acid residues are shown in black, whereas nonconserved residues are shown in gray. Two Laminin EGF-like repeats, each containing eight conserved cysteine residues (shaded), are marked by arrows. Aligned sequences of various Net and Laminin proteins are shown in (B). The portion of the afrNet sequence aligned corresponds to the region used to generate the anti-afrNet antibody. Amino acid residue numbers for the proteins used in this study are indicated. This alignment was used to generate a phylogenetic tree in Phylip (C), which supports placement of afrNet among the Net proteins. Bootstrap values for each clade are shown as percentages.