Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Proteome Res. 2009 Feb;8(2):631–642. doi: 10.1021/pr800758g

Figure 3.

Figure 3

(A.) ETD of the precursor ion m/z 788.2 (2+), corresponding to the peptide 289-302 of the full length APP695 containing the monosaccharide N-acetyl galactosamine (yellow rectangle) attached at Thr 292. The red label indicates the radical loss of 219Da (·OGalNAc) from the radical species [M+2H]+· and z11·, respectively. The fragment ions relevant for determination of the glycosylation site are indicated with black boxes. The inset in this spectrum shows the peak distribution of the species z7, z10 and z12 (see discussion in text). The spectrum was obtained using data dependent acquisition without supplemental ion activation. (B) Inset over the mass range m/z 1510 - 1580, showing the small neutral losses derived from the molecule radical ion of m/z 1576.4.