Table 1.
Glycoform | Experimental MW (Da) | Theoretical MW (Da) | Retention time (min) | Occupied sites observed in peptide 289 - 302 |
|
---|---|---|---|---|---|
Thr 291 | Thr 292 | ||||
a | 1371.3 | 1371.69 | 20.8 | - | - |
b | 1574.3 | 1574.77 | 20.6 | - | GalNAc |
c | 1736.2 | 1736.82 | 20.4 | - | GalNAcGal |
d | 1777.5 | 1777.85 | 20.4 | GalNAc | GalNAc |
e | 1939.4 | 1939.90 | 19.8 | GalNAc | GalNAcGal |
f | 2027.3 | 2027.92 | 20.7 | - | GalNAcGalSA |
g | 2101.4 | 2101.95 | 19.5 | GalNAcGal | GalNAcGal |
h | 2230.3 | 2230.99 | 20.3 | GalNAc | GalNAcGalSA |
i | 2392.3 | 2393.05 | 20.1 | GalNAcGal | GalNAcGalSA |
j | 2683.3 | 2684.14 | 20.2 | GalNAcGalSA | GalNAcGalSA |