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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Cell Death Differ. 2009 Jan 16;16(5):684–696. doi: 10.1038/cdd.2008.195

Figure 1. Puma and Noxa expression is increased in B cells from Eμ-myc mice.

Figure 1

(a) Differences in the levels of RNA for Bcl-2 family members and Myc between cells from Eμ-myc and non-transgenic mice. Pro-B, pre-B and B-cell subsets were FACS purified from healthy 5- to 6-week-old Eμ-myc or non-transgenic syngeneic (C57BL/6) mice. SYBR green real-time PCR analysis was performed on cDNA. Relative RNA expression levels were calculated by normalising to the signal for β-actin in each sample and then dividing the transgenic by the non-transgenic value. Mean expression is shown ± S.E.M. of cells from 3–4 individual mice of each genotype from at least three separate experiments. (b) Western blot analysis of Puma and β-actin (loading control) on protein isolated from the cell populations described in A. Puma-deficient thymocytes were included as a control for antibody specificity. Protein size standards in kDa are indicated on the left