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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Cell Death Differ. 2009 Jan 16;16(5):684–696. doi: 10.1038/cdd.2008.195

Figure 7. Tumours from Eμ-myc/noxa−/−puma+/− mice retain the wt puma allele but some exhibit reduced levels of Puma expression.

Figure 7

Allele-specific PCR for retention of the residual wt puma allele was performed on (a) 12 randomly selected tumours from Eμ-myc/noxa−/−puma+/− mice and (b) lymphoma cells sorted from four Eμ-myc/noxa−/−puma+/− mice based on large forward scatter and staining for B220. The wt and knockout puma alleles are 203 and 376 bp, respectively. A positive control for puma heterozygosity (+ve) and a no-DNA control (−ve) were included. (c) Western blot analysis of Puma and β-actin (loading control) in randomly selected Eμ-myc/noxa−/−puma+/− tumours. One Eμ-myc/noxa−/−puma+/− tumour (no. 33) is repeated as a loading control alongside two tumours (PM211, PM291) from Eμ-myc/puma−/− mice, in which no Puma could be detected. The anti-Puma antibody also detects a non-specific band (ns). Protein size standards in kDa are indicated on the left