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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2006 Mar–Apr;42(3-4):96–99. doi: 10.1290/0512083.1

FIG. 2.

FIG. 2

Supershift assay showing c-Jun and c-Fos as active constituents of the AP-1 complex in nuclear extract obtained from frontal cortex. Nuclear extract was incubated in presence or absence of antibodies as indicated. To validate the specificity of the assay, the binding reaction was performed in presence of nonspecific antibody Rel A, preimmune serum, and 1000-fold excess of unlabeled oligonucleotide. The gel was dried, and bound complex was detected by exposing the gel to a PhosphorImager.