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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2006 Mar–Apr;42(3-4):96–99. doi: 10.1290/0512083.1

FIG. 3.

FIG. 3

Simulated microgravity activates JNK-1 kinase activity in different regions of the brain. JNK-1 was immunoprecipitated from different regions of the brain tissue extracts, and then kinase assay was performed in presence of [γ32P] ATP and GST-Jun (1–79) substrate. The reaction was terminated by adding 2×SDS sample buffer, boiled for 5 min, and subjected to SDS-PAGE (9%). The gel was dried, and c-Jun phosphorylation was detected by exposing the gel to PhosphorImager.