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. Author manuscript; available in PMC: 2009 Sep 15.

Published in final edited form as: Bioconjug Chem. 2006 Nov–Dec;17(6):1545–1550. doi: 10.1021/bc060154p

Table 2.

Analytical Data for Synthetic Compounds

Ac-NDP-α-MSH-linker-NDP-α-MSH-NH₂					Ac-linker-NDP-α-MSH-NH₂
	mass spectrum^a			HPLC^b		mass spectrum^a			HPLC^b
compd	ion	calcd	obsd	k′	compd	ion	calcd	obsd	k′
9	(M + 3)³⁺	1122.896	1122.90	18.13	12	(M + 2)²⁺	890.4446	890.40	15.11
10	(M + 2)²⁺	1721.860	1721.81	20.24	13	(M + 2)²⁺	995.4462	995.40	22.69
11	(M + 3)³⁺	1173.592	1173.50	22.43	14	(M + 2)²⁺	993.4618	993.91	27.11
32	(M + 4)⁴⁺	873.9417	873.94	21.35	33	(M + 3)³⁺	653.9693	653.98	25.25
Ac-MSH(4)-linker-MSH(4)-NH₂					Ac-linker-MSH(4)-NH₂
	mass spectrum^a			HPLC^b		mass spectrum^a			HPLC^b
compd	ion	calcd	obsd	k′	compd	ion	calcd	obsd	k′

37	(M + 1)¹⁺	1445.705	1445.911	9.16	41	(M + 1)¹⁺	819.3976	819.40	7.05
38	(M + 1)¹⁺	1521.737	1522.176	10.77	42	(M + 1)¹⁺	895.4289	895.41	9.65
39	(M + 1)¹⁺	1597.768	1597.910	13.00	43	(M + 1)¹⁺	971.4603	971.65	13.13
40	(M + 1)¹⁺	1571.752	1571.833	12.03	44	(M + 1)¹⁺	945.4445	945.48	11.66

^a

MS found molecular peaks (M + 1)¹⁺, (M + 2)²⁺, (M + 3)³⁺, and (M + 4)⁴⁺. One diagnostic peak is listed here for each compound.

^b

Peptides were eluted with a linear MeCN/0.1% CF₃CO₂H aqueous gradient (10% to 40% in 30 min) at a flow rate of 1.0 mL/min.