Skip to main content
. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Chem Phys Lipids. 2009 Jun 13;161(1):38–43. doi: 10.1016/j.chemphyslip.2009.06.140

Fig. 2.

Fig. 2

Inhibition of relaxation activity of recombinant LdTopIB by compound 1. One unit of recombinant LdTop1 was assayed in a plasmid DNA relaxation assay for 30 min at 37 °C (as described under “Material and Methods”) in the presence of 12.5 to 1000 μM compound 1. Reaction products were resolved in agarose gel and subsequently visualized by ethidium bromide staining. The relative position of the negatively supercoiled DNA substrate is indicated by Sc, N is the nicked DNA, whereas the ladder of relaxed DNA topoisomer bands is labeled R. Reactions were stopped with a mixture of 1 % SDS and 6.1 μg of proteinase K. Lane 1 contains 0.2 mg of pHOT plasmid DNA and lane 2 is a relaxed marker.