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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Nature. 2009 Feb 22;458(7235):172–177. doi: 10.1038/nature07836

Fig. 3.

Fig. 3

3-D reconstruction of an ESCRT-III treated GUV. a, Three-dimensional reconstruction of a GUV that was incubated with Vps20ΔC, Snf7, Vps24, Vps2, and Vps4, with GFP added together with Snf7. b, A Z stack of the same GUV shows that the ILVs are filled with GFP indicating that they were detached subsequent to the addition of Snf7. Scale bar = 5 μm.