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. 2009 Sep 23;4(9):e7137. doi: 10.1371/journal.pone.0007137

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Wodarczyk et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) Different tissues from homozygous Pkd1^HA/HA mice (P2 and P20) were isolated and analyzed by IP and western blots. (A) Different exposure times of the same western blot revealed that the expression of PC-1 is very low in kidney, moderate in the brain, heart, liver and spleen, and very high in the lung. (B) PC-1 expression levels were compared in lung and brain at P2 and P20 by IP/western blot. Whereas PC-1 is constantly expressed at high levels in the lung, in the brain the expression levels decrease between days 2 and 20 of postnatal life of the mouse. (C–E) Brains of homozygous as well as wild-type litter mates from P2 were isolated, dissected, and immunostained with anti-HA antibodies for detection of HA-tagged PC1 (red) and acetylated α-tubulin for labelling the cilia (green). Figure 4C shows the staining of ependymal multiciliated cells with anti-HA antibodies. (D, E) Both monociliated (D) and multiciliated (E) choroid plexus cilia stained positive with anti-HA antibodies. Scale bars: C,D 5 µm; E 2 µm.