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Immunocytochemistry (× 400). A: In negative control, the primary antibody was omitted; B and E: PI 3-K p85 and p-Akt473 staining in the PDGF-BB group; C and F: PI 3-K p85 and p-Akt473 expression in the PDGF-BB and LY 294002 groups; D and G: PI 3-K p85 and p-Akt473 staining in the LY 294002 group. Rabbit anti-PI 3-K p85α polyclonal antibody (1:50) and rabbit anti-phospho-Ser473 Akt polyclonal antibody (1:100) used as primary antibodies.
