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. Author manuscript; available in PMC: 2010 May 26.
Published in final edited form as: Circulation. 2009 May 11;119(20):2686–2692. doi: 10.1161/CIRCULATIONAHA.108.790485

Figure 1. Expression of Notch receptors in endothelial and smooth muscle cells.

Figure 1

A. Western blot analysis shows that the expression of total Notch1 in EC isolated from hearts of WT, Notch1flox/+, smCre-Tg, N1+/-, and smN1+/- mice.

B. Western blot analysis shows that the expression of total Notch1 and Notch3 in SMC isolated from aortas of WT, Notch1flox/+, smCre-Tg, N1+/-, and smN1+/- mice.

C. Immunofluorescence staining of Notch1, Notch2, and Notch3 in uninjured carotid artery (left panel) and ligated injured carotid artery (14 days after ligation, right panel) of WT mice. Vessels were co-stained for smooth muscle actin (SMA). SMA is stained red; Notch receptors were stained green; and nuclei were stained blue (DAPI). Yellow indicates presence of both red and green staining. White bar was 20 μm.

D. Immunofluorescence co-staining of smooth muscle actin (SMA) with activated Notch1 (NICD). Red was SMA, green was NICD, and blue was DAPI. White bar was 20 μm.