Skip to main content
. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Virus Res. 2009 Jan 29;142(1-2):92–99. doi: 10.1016/j.virusres.2009.01.014

Figure 1. Chemiluminescent immunodetection of henipavirus infection in various cell lines.

Figure 1

Cell lines were infected with HeV (left panel) and NiV (right panel) and viral nucleoprotein was detected in using a previously described HTS format (Aljofan et al., 2008). ½ log dilutions of virus (100μl) were incubated for 24 hours at 37 °C with 20,000 cells per well. Monolayers were fixed with methanol, air dried and immunostained with anti-NiV-N polyclonal antisera (1:1000) followed by secondary antibodies (1:2000) of Horse Radish Peroxidase conjugate with chemiluminescent (HRP-CL) detection (n=4). Values are expressed as the Mean +/− S.E.