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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Virus Res. 2009 Jan 29;142(1-2):92–99. doi: 10.1016/j.virusres.2009.01.014

Figure 4. Infectious titers following NiV infection in various cell lines.

Figure 4

Cells were infected with 10,000 TCID50/ml NiV in 100μl for 60 min at 37 °C. Inoculum was replaced with fresh EMEM-10 and cells were incubated overnight prior to titration of cell supernatants via serial ten-fold dilutions made in EMEM-10 in 96-well microtitre plates. Vero E6 cells in EMEM-10 were added (2 × 104 cells/well) and plates were incubated at 37 °C for 5–7 days. Wells displaying cytopathic effect were scored as infected and virus titer was calculated using the Reed-Meunch method (Reed and Muench, 1938).