(A) Structural formulas of neutral lipids formed upon sterol biosynthesis (1-squalene; 2-squalene epoxide; 3-lanosterol; 4 - 24-methylenedihydrolanosterol; 5 – obtusifoliol; 6- ergosterol; 7- 24- methylergosterol) or up taken from the media (8-cholesterol). 14α-methyl group and formed by CYP51 Δ14–15 double bond are shown in red.
(B) TLC analysis of sterols extracted from TC epimastigotes. St, sterol standards; C, sterols in untreated cells; T, sterols in TC incubated for 24 hours with 50 μM 20.