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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Cancer Res. 2009 Jun 16;7(6):841–850. doi: 10.1158/1541-7786.MCR-08-0555

Figure 4.

Figure 4

Hag mutations are insertions in the fbxw4 gene but affect expression of the neighboring fgf8 gene. A. Location of the mutagenic insertions in Hag mutants. hiD2058 is precisely at the splice donor of exon 1 of fbxw4; the other three insertions are in the 24 kb fifth intron. Enhancer trap insertions CLGY1030 and CLGY508 are in intergenic sequence upstream and downstream of fgf8, respectively. B. fbxw4 and fgf8 mRNA measured in whole fish by real-time RT-PCR in wild type and homozygotes of multiple Hag alleles at 5 and 41 days. Units are normalized to wild type sample of each developmental age. C. fgf8 mRNA measured in whole fish by real-time RT-PCR in wild type and hiD4000 homozygotes from 3 to 41 days. Units are normalized to the 3 day wild type sample. For B and C, error bars show the standard deviation of 2−3 technical replicates.